In this study we have measured the intensity of serum emission at 450 nm (emission of NAD(P)H) for patients with chronic fatigue syndrome (CFS). Changes in emission of serum in UV-visible range for patients with CFS are compared to other laboratory tests. Results of our analysis demonstrated that: -Bioenergetics measured by serum emission averaged 20% lower in patients than controls. Serum emission intensity correlated with the concentration of coenzyme Q in serum. Antibodies to infections Candida albicans and Epstein-Barr virus (EBV) were elevated in 71%-85% of patients.

There was no correlation between the level of serum emission or energy metabolism and the level of antibodies in serum.

Fatigue was not caused by an under active thyroid; 75% of patents had hormone free T3 in normal range. Pyrroles concentration in urine was higher than the normal range and correlated inversely with serum emission (R=0.6).